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验证数据展示

  • WB analysis using 81435-2-RR (same clone as 81435-2-PBS)

    WB analysis using 81435-2-RR (same clone as 81435-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 81435-2-RR (same clone as 81435-2-PBS)

    WB analysis of NIH/3T3 using 81435-2-RR (same clone as 81435-2-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 81435-2-RR (same clone as 81435-2-PBS)

    WB analysis of MCF-7 using 81435-2-RR (same clone as 81435-2-PBS)

    WB result of USP10 antibody (81435-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP10 transfected MCF-7 cells. This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81435-2-RR (same clone as 81435-2-PBS)

    IF Staining of HepG2 using 81435-2-RR (same clone as 81435-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP10 antibody (81435-2-RR, Clone: 240583G6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

USP10 Recombinant monoclonal antibody, PBS Only

USP10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81435-2-PBS

产品说明书

CloneNo. 240583G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

240583G6, Deubiquitinating enzyme 10, EC:3.4.19.12, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

81435-2-PBS targets USP10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7233

Product name: Recombinant human USP10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 444-798 aa of BC000263

Sequence: KFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 10
别名 240583G6, Deubiquitinating enzyme 10, EC:3.4.19.12, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10
计算分子量 87 kDa, 92 kDa
观测分子量 100-130 kDa
GenBank蛋白编号BC000263
基因名称 USP10
Gene ID (NCBI) 9100
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ14694
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.

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