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验证数据展示

  • WB analysis using 19374-1-AP (same clone as 19374-1-PBS)

    WB analysis using 19374-1-AP (same clone as 19374-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19374-1-AP (USP10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19374-1-PBS in a different storage buffer formulation.

  • WB analysis using 19374-1-AP (same clone as 19374-1-PBS)

    WB analysis using 19374-1-AP (same clone as 19374-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19374-1-AP (USP10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19374-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 19374-1-AP (same clone as 19374-1-PBS)

    IP experiment of HEK-293 using 19374-1-AP (same clone as 19374-1-PBS)

    IP result of anti-USP10 (IP:19374-1-AP, 4ug; Detection:19374-1-AP 1:500) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 19374-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 19374-1-AP (same clone as 19374-1-PBS)

    IHC staining of human breast cancer using 19374-1-AP (same clone as 19374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19374-1-AP (USP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19374-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 19374-1-AP (same clone as 19374-1-PBS)

    IHC staining of human breast cancer using 19374-1-AP (same clone as 19374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19374-1-AP (USP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19374-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 19374-1-AP (same clone as 19374-1-PBS)

    IF Staining of HEK-293 using 19374-1-AP (same clone as 19374-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using USP10 antibody (19374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19374-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

USP10 Polyclonal antibody, PBS Only

USP10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 19374-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Deubiquitinating enzyme 10, EC:3.4.19.12, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10, Ubiquitin-specific-processing protease 10

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

19374-1-PBS targets USP10 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7233

Product name: Recombinant human USP10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 444-798 aa of BC000263

Sequence: KFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 10
别名 Deubiquitinating enzyme 10, EC:3.4.19.12, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10, Ubiquitin-specific-processing protease 10
计算分子量 87 kDa, 92 kDa
观测分子量 100-130 kDa
GenBank蛋白编号BC000263
基因名称 USP10
Gene ID (NCBI) 9100
RRIDAB_10858617
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14694
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.

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