验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19374-1-AP targets USP10 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7233 Product name: Recombinant human USP10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 444-798 aa of BC000263 Sequence: KFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 10 |
| 别名 | Deubiquitinating enzyme 10, EC:3.4.19.12, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10, Ubiquitin-specific-processing protease 10 |
| 计算分子量 | 87 kDa, 92 kDa |
| 观测分子量 | 100-130 kDa |
| GenBank蛋白编号 | BC000263 |
| 基因名称 | USP10 |
| Gene ID (NCBI) | 9100 |
| RRID | AB_10858617 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14694 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for USP10 antibody 19374-1-AP | Download protocol |
| IP protocol for USP10 antibody 19374-1-AP | Download protocol |
| WB protocol for USP10 antibody 19374-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. | ||
Mol Cell Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance. | ||
Cell Death Differ Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma
| ||
Nat Commun Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.
| ||
J Cell Biol High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate. | ||
