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USP10 Polyclonal antibody
USP10 Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IF detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 19374-1-AP is USP10 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | USP10 fusion protein Ag7233 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin specific peptidase 10 |
| Synonyms | Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10 |
| Calculated Molecular Weight | 87 kDa, 92 kDa |
| Observed Molecular Weight | 100-130 kDa |
| GenBank Accession Number | BC000263 |
| Gene Symbol | USP10 |
| Gene ID (NCBI) | 9100 |
| RRID | AB_10858617 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14694 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP10 antibody 19374-1-AP | Download protocol |
| IF protocol for USP10 antibody 19374-1-AP | Download protocol |
| IP protocol for USP10 antibody 19374-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. | ||
Mol Cell Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance. | ||
Cell Death Differ Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma
| ||
Nat Commun Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.
| ||
J Cell Biol High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate. | ||
