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UPK1B Polyclonal antibody

UPK1B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 26853-1-AP

Print datasheet

Synonyms

Tetraspanin 20, Tspan 20, TSPAN20, UP1b, UPIB, UPK1, UPK1B, uroplakin 1B, Uroplakin Ib

验证数据展示

  • IHC staining of human urothelial carcinoma using 26853-1-AP

    IHC staining of human urothelial carcinoma using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 26853-1-AP

    IHC staining of human urothelial carcinoma using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman urothelial carcinoma tissue, mouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26853-1-AP targets UPK1B in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA
Tested Reactivity human, mouse
Immunogen UPK1B fusion protein Ag25471 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name uroplakin 1B
Synonyms Tetraspanin 20, Tspan 20, TSPAN20, UP1b, UPIB, UPK1, UPK1B, uroplakin 1B, Uroplakin Ib
GenBank Accession NumberBC063568
Gene Symbol UPK1B
Gene ID (NCBI) 7348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75841
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1B (UPK1B) belongs to the tetraspanin (TM4SF) family. UPK1B can promote the proliferation, invasion and metastasis of tumor cells and regulate the development and progression of tumors (PMID: 30229818).

实验方案

Product Specific Protocols
IHC protocol for UPK1B antibody 26853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols