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CoraLite® Plus 488-conjugated UPF1 Monoclonal antibody

UPF1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B6B5

Cat No : CL488-66898

Print datasheet

Synonyms

ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1

验证数据展示

  • IF Staining of HepG2 using CL488-66898

    IF Staining of HepG2 using CL488-66898

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 UPF1 antibody (CL488-66898, Clone: 3B6B5 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66898

    FC experiment of HepG2 using CL488-66898

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human UPF1 (CL488-66898, Clone:3B6B5) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66898-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66898 targets UPF1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen UPF1 fusion protein Ag28320 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Synonyms ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1
Calculated Molecular Weight123 kDa
Observed Molecular Weight 123-130 kDa
GenBank Accession NumberBC039817
Gene Symbol UPF1
Gene ID (NCBI) 5976
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92900
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UPF1 antibody CL488-66898Download protocol
FC protocol for CL Plus 488 UPF1 antibody CL488-66898Download protocol
Standard Protocols
Click here to view our Standard Protocols