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验证数据展示

  • WB analysis using 67249-1-Ig

    WB analysis using 67249-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67249-1-Ig (ULBP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

ULBP2 Monoclonal antibody

ULBP2 Monoclonal Antibody for WB, ELISA
Cat No. 67249-1-Ig

产品说明书

CloneNo. 1A9G8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

ALCAN alpha, N2DL 2, N2DL2, NKG2D ligand 2, NKG2DL2, RAET1H, UL16 binding protein 2, ULBP2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, TF-1 cells, K-562 cells, THP-1 cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy ULBP2 Ready-To-Use IHC Kit. ULBP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67249-1-Ig targets ULBP2 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag28879

Product name: Recombinant human ULBP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-224 aa of BC034689

Sequence: AGRADPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGNKTVTPVSPLGKKLNVTTAWKAQNPVLREVVDILTEQLRDIQLENYTPKEPLTLQARMSCEQKAEGHSSGSWQFSFDGQIFLLFDSEKRMWTTVHPGARKMKEKWENDKVVAMSFHYFSMGDCIGWLEDFLMGMDSTLEPSAGAPLAMSSGTTQLRA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 UL16 binding protein 2
别名 ALCAN alpha, N2DL 2, N2DL2, NKG2D ligand 2, NKG2DL2, RAET1H, UL16 binding protein 2, ULBP2
计算分子量 246 aa, 27 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC034689
基因名称 ULBP2
Gene ID (NCBI) 80328
RRIDAB_2882526
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BZM5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKG2D is an activating cell surface receptor that is predominantly expressed on cytotoxic immune cells. NKG2D recognizes a wide range of ligands. In humans, the NKG2D ligand (NKG2DL) includes MICA, MICB, and six members of the ULBP family (ULBP1-6) (PMID: 29568297; 30813924). ULBPs are MHC class I-related molecules (PMID: 11239445). ULBP2 contains two Ig-like domains, the alpha-1 and alpha-2 domains characteristic of the MHC class I family, but lacks the alpha-3 domain. It can be expressed as either a transmembrane or GPI-linked protein, or released from the cell surface (PMID: 21224393; 24614922). ULBP2 binds and activates the NKG2D, mediating the recruitment and activation of NK cells.

实验方案

Product Specific Protocols
WB protocol for ULBP2 antibody 67249-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols