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UBQLN4 Polyclonal antibody

UBQLN4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29413-1-AP

Print datasheet

Synonyms

A1U, C1orf6, CIP75, UBIN, ubiquilin 4, UBQLN4

验证数据展示

  • WB analysis using 29413-1-AP

    WB analysis using 29413-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29413-1-AP (UBQLN4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 29413-1-AP

    IHC staining of human cervical cancer using 29413-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29413-1-AP (UBQLN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 29413-1-AP

    IHC staining of human cervical cancer using 29413-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29413-1-AP (UBQLN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 29413-1-AP

    IHC staining of human cervical cancer using 29413-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29413-1-AP (UBQLN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inBGC-823 cells, BxPC-3 cells, HeLa cells, MKN-45 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29413-1-AP targets UBQLN4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen UBQLN4 fusion protein Ag29658 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquilin 4
Synonyms A1U, C1orf6, CIP75, UBIN, ubiquilin 4, UBQLN4
Calculated Molecular Weight 601 aa, 64 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC063841
Gene Symbol UBQLN4
Gene ID (NCBI) 56893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRR5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquilins (UBQLNs) are important factors for cell proteostasis maintenance. UBQLNs are involved in the modulation of the cell cycle, as well as in apoptosis, membrane receptors regulation, DNA repair, epithelial-mesenchymal transition, and miRNA activities. Ubiquilin 4 (UBQLN4) is an important member of the ubiquitin-like protein family. UBQLN4 is overexpressed in aggressive tumors and inhibits homologous recombination, redirecting doublestrand break repair to nonhomologous end joining, resulting in increased genome instability and inducing carcinogenesis.

实验方案

Product Specific Protocols
WB protocol for UBQLN4 antibody 29413-1-APDownload protocol
IHC protocol for UBQLN4 antibody 29413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols