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验证数据展示

  • WB analysis using 29413-1-AP

    WB analysis using 29413-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29413-1-AP (UBQLN4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 29413-1-AP

    IHC staining of human cervical cancer using 29413-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29413-1-AP (UBQLN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 29413-1-AP

    IHC staining of human cervical cancer using 29413-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29413-1-AP (UBQLN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 29413-1-AP

    IHC staining of human cervical cancer using 29413-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29413-1-AP (UBQLN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBQLN4 Polyclonal antibody

UBQLN4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29413-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

A1U, C1orf6, CIP75, UBIN, ubiquilin 4, UBQLN4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBGC-823 cells, BxPC-3 cells, HeLa cells, MKN-45 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29413-1-AP targets UBQLN4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 UBQLN4 fusion protein Ag29658 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquilin 4
别名 A1U, C1orf6, CIP75, UBIN, ubiquilin 4, UBQLN4
计算分子量 601 aa, 64 kDa
观测分子量70 kDa
GenBank蛋白编号BC063841
基因名称 UBQLN4
Gene ID (NCBI) 56893
RRIDAB_3086129
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRR5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquilins (UBQLNs) are important factors for cell proteostasis maintenance. UBQLNs are involved in the modulation of the cell cycle, as well as in apoptosis, membrane receptors regulation, DNA repair, epithelial-mesenchymal transition, and miRNA activities. Ubiquilin 4 (UBQLN4) is an important member of the ubiquitin-like protein family. UBQLN4 is overexpressed in aggressive tumors and inhibits homologous recombination, redirecting doublestrand break repair to nonhomologous end joining, resulting in increased genome instability and inducing carcinogenesis.

实验方案

Product Specific Protocols
WB protocol for UBQLN4 antibody 29413-1-APDownload protocol
IHC protocol for UBQLN4 antibody 29413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols