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验证数据展示

  • WB analysis of HeLa using 60038-1-Ig (same clone as 60038-1-PBS)

    WB analysis of HeLa using 60038-1-Ig (same clone as 60038-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60038-1-Ig (UBE3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60038-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 60038-1-Ig (same clone as 60038-1-PBS)

    IHC staining of human kidney using 60038-1-Ig (same clone as 60038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60038-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE3A antibody included
  • IHC staining of human kidney using 60038-1-Ig (same clone as 60038-1-PBS)

    IHC staining of human kidney using 60038-1-Ig (same clone as 60038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60038-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE3A antibody included
  • Sample test of MP51408-1

    Sample test of MP51408-1

    Sample test of MP51408-1, UBE3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60038-1-PBS. Detection antibody: 60977-1-PBS.

  • Cytometric bead array standard curve of MP51408-1

    Cytometric bead array standard curve of MP51408-1

    Cytometric bead array standard curve of MP51408-1, UBE3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60038-1-PBS. Detection antibody: 60977-1-PBS. Standard:Ag17546. Range: 3.125-400 ng/mL .

UBE3A Monoclonal antibody, PBS Only (Capture)

UBE3A Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 60038-1-PBS

产品说明书

CloneNo. 3D6H9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

别名

3D6H9, ANCR, AS, E6 AP, E6AP

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy UBE3A Ready-To-Use IHC Kit. UBE3A primary antibody included.

产品信息

60038-1-PBS targets UBE3A as part of a matched antibody pair:

MP51408-1: 60038-1-PBS capture and 60977-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0346

Product name: Recombinant human UBE3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 9-215 aa of BC002582

Sequence: GEPQSDDIEASRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARIDFKDVTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVKQHTKEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin protein ligase E3A
别名 3D6H9, ANCR, AS, E6 AP, E6AP
计算分子量 852 aa, 98 kDa
观测分子量 100 kDa
GenBank蛋白编号BC002582
基因名称 UBE3A
Gene ID (NCBI) 7337
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ05086
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE3A(Ubiquitin-protein ligase E3A) is also named as E6AP, EPVE6AP, HPVE6A and belongs to the E3 ubiquitin protein ligase family. It functions as both an E3 ligase in the ubiquitin proteasome pathway and as a transcriptional coactivator. It is also initially identified as a cellular protein that mediates in vitro association of the human papillomavirus E6 protein with p53, leading to the ubiquitin-dependent degradation of p53(PMID:1661671). Defects in UBE3A are a cause of Angelman syndrome (AS)(PMID:10508479 ). It has 3 isoforms produced by alternative splicing.