• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of A431 using 12581-1-AP

    WB analysis of A431 using 12581-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 12581-1-AP

    IHC staining of human skin cancer using 12581-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 12581-1-AP (UBE2Q2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin cancer using 12581-1-AP

    IHC staining of human skin cancer using 12581-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 12581-1-AP (UBE2Q2 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of HEK-293T using 12581-1-AP

    WB analysis of HEK-293T using 12581-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293T using 12581-1-AP

    IP experiment of HEK-293T using 12581-1-AP

    IP result of anti-UBE2Q2 (IP:12581-1-AP, 4ug; Detection:12581-1-AP 1:300) with HEK-293T cells lysate 1320 ug.

UBE2Q2 Polyclonal antibody

UBE2Q2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12581-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

UBCi, UBE2Q2, Ubiquitin carrier protein Q2, Ubiquitin protein ligase Q2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, mouse ovary tissue, HEK-293T cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12581-1-AP targets UBE2Q2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 UBE2Q2 fusion protein Ag3276 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2Q family member 2
别名 UBCi, UBE2Q2, Ubiquitin carrier protein Q2, Ubiquitin protein ligase Q2
计算分子量 375 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC017708
基因名称 UBE2Q2
Gene ID (NCBI) 92912
RRIDAB_2256807
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE2Q2 antibody 12581-1-APDownload protocol
IHC protocol for UBE2Q2 antibody 12581-1-APDownload protocol
IP protocol for UBE2Q2 antibody 12581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
View Article
mouseWB

Transl Cancer Res

Proteome-wide identification and functional analysis of ubiquitinated proteins in Hepa1-6 cells by knockdown of E3 ubiquitin ligase SIAH1

Authors - Xiao Hu
View Article