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UBE2H Polyclonal antibody

UBE2H Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15685-1-AP

Print datasheet

Synonyms

E2 20K, UBC8, UBCH, UBCH2, UBE2H, Ubiquitin carrier protein H, Ubiquitin protein ligase H

验证数据展示

  • WB analysis using 15685-1-AP

    WB analysis using 15685-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 15685-1-AP

    IHC staining of human colon using 15685-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 15685-1-AP (UBE2H Antibody) at dilution of 1:50.

  • IHC staining of human colon using 15685-1-AP

    IHC staining of human colon using 15685-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 15685-1-AP (UBE2H Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse kidney tissue, HeLa cells, rat brain tissue, rat kidney tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15685-1-AP targets UBE2H in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen UBE2H fusion protein Ag8187 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
Synonyms E2 20K, UBC8, UBCH, UBCH2, UBE2H, Ubiquitin carrier protein H, Ubiquitin protein ligase H
Calculated Molecular Weight 183 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC006277
Gene Symbol UBE2H
Gene ID (NCBI) 7328
RRIDAB_10858632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62256
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE2H antibody 15685-1-APDownload protocol
IHC protocol for UBE2H antibody 15685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols