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  • Featured Product
  • KD/KO Validated

UBE2G1 Polyclonal antibody

UBE2G1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12012-1-AP

Print datasheet

Synonyms

E217K, UBC7, UBE2G, UBE2G1, Ubiquitin carrier protein G1, Ubiquitin protein ligase G1

验证数据展示

  • WB analysis of HeLa using 12012-1-AP

    WB analysis of HeLa using 12012-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12012-1-AP (UBE2G1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12012-1-AP

    IF Staining of HepG2 using 12012-1-AP

    Immunofluorescent analysis of HepG2 cells, using UBE2G1 antibody 12012-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12012-1-AP targets UBE2G1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen UBE2G1 fusion protein Ag2625 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast)
Synonyms E217K, UBC7, UBE2G, UBE2G1, Ubiquitin carrier protein G1, Ubiquitin protein ligase G1
Calculated Molecular Weight 170 aa, 19 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC026288
Gene Symbol UBE2G1
Gene ID (NCBI) 7326
RRIDAB_10665812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62253
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE2G1 antibody 12012-1-APDownload protocol
IF protocol for UBE2G1 antibody 12012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Genes Dev

Sequential replication-coupled destruction at G1/S ensures genome stability.

Authors - Kate E Coleman
  • KD Validated
View Article
humanWB

Elife

Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation.

Authors - Lu Lv
View Article