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验证数据展示

  • WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 16081-1-AP (same clone as 16081-1-PBS)

    IHC staining of human ovary tumor using 16081-1-AP (same clone as 16081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WARS antibody included
  • IHC staining of human ovary tumor using 16081-1-AP (same clone as 16081-1-PBS)

    IHC staining of human ovary tumor using 16081-1-AP (same clone as 16081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WARS antibody included
  • WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    IP experiment of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    IP result of anti-TrpRS (IP:16081-1-AP, 4ug; Detection:16081-1-AP 1:500) with HepG2 cells lysate 3000ug. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    IF Staining of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using TrpRS antibody (16081-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    FC experiment of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TrpRS Polyclonal antibody (16081-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TrpRS Polyclonal antibody, PBS Only

TrpRS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 16081-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

WARS, Interferon-induced protein 53, IFP53, IFI53, hWRS

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.

产品信息

16081-1-PBS targets TrpRS in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9079

Product name: Recombinant human WARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-368 aa of BC017489

Sequence: MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tryptophanyl-tRNA synthetase
别名 WARS, Interferon-induced protein 53, IFP53, IFI53, hWRS
计算分子量 471 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC017489
基因名称 WARS1
Gene ID (NCBI) 7453
RRIDAB_2273061
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23381
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TrpRS is also named as IFI53, WRS, WARS and belongs to the class-I aminoacyl-tRNA synthetase family. It catalyzes activation of tryptophan by ATP and transfer to tRNATrP, ensuring ranslation of the genetic code for tryptophan. TrpRS is thought to be more closely related to TyrRS than to any other class I enzyme, but the significance of this relationship has been questioned (PMID:7743129). It can exsit as a dimer(PMID:7743129). It has 2 isoforms produced by alternative splicing.

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