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验证数据展示

  • WB analysis using 21685-1-AP (same clone as 21685-1-PBS)

    WB analysis using 21685-1-AP (same clone as 21685-1-PBS)

    Mouse small intestine tissue lysate were subjected to SDS PAGE followed by western blot with 21685-1-AP (Trehalase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21685-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 21685-1-AP (same clone as 21685-1-PBS)

    IF Staining of human kidney using 21685-1-AP (same clone as 21685-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Trehalase antibody (21685-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21685-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 21685-1-AP (same clone as 21685-1-PBS)

    IF Staining of human kidney using 21685-1-AP (same clone as 21685-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Trehalase antibody (21685-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21685-1-PBS in a different storage buffer formulation.

Trehalase Polyclonal antibody, PBS Only

Trehalase Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 21685-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, Indirect ELISA

别名

TREH, Alpha,alpha trehalase, Alpha,alpha-trehalase, Alpha,alpha-trehalose glucohydrolase, EC:3.2.1.28

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21685-1-PBS targets Trehalase in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16246

Product name: Recombinant human TREH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 203-552 aa of BC109206

Sequence: YLTHTNDTAFLQENIETLALELDFWTKNRTVSVSLEGKNYLLNRYYVPYGGPRPESYSKDVELADTLPEGDREALWAELKAGAESGWDFSSRWLIGGPNPNSLSGIRTSKLVPVDLNAFLCQAEELMSNFYSRLGNDSQATKYRILRSQRLAALNTVLWDEQTGAWFDYDLEKKKKNREFYPSNLTPLWAGCFSDPGVADKALKYLEDNRILTYQYGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRTNFDVYSQKSAMYEKYDVSNGGQPGGGGEYEVQEGFGWTNGVVLMLLDRYGDRLTSGAKLAFLEPHCLAATLLPSLLLSLLPW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trehalase (brush-border membrane glycoprotein)
别名 TREH, Alpha,alpha trehalase, Alpha,alpha-trehalase, Alpha,alpha-trehalose glucohydrolase, EC:3.2.1.28
计算分子量 583 aa, 67 kDa
观测分子量67-70 kDa
GenBank蛋白编号BC109206
基因名称 Trehalase
Gene ID (NCBI) 11181
RRIDAB_3742050
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43280
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Trehalase is the enzyme hydrolyzing trehalose and belongs to the glycosyl hydrolase 37 family. In humans and most mammals, trehalase is found in the brush borders of the intestinal mucosa, kidney, liver, and blood plasma. Trehalases are conserved enzymes that catalyze the hydrolysis of one of two glycoside bonds of trehalose. With the exception of vertebrates where trehalase is required for the degradation of ingested trehalose, in other animal groups it is involved in diverse physiological processes including carbohydrate metabolism, resumption of growth in certain resting cells, germination of spores in fungi, recovery from stress conditions, etc (PMID: 25429048, PMID: 30628830).

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