验证数据展示
经过测试的应用
| Positive WB detected in | mouse small intestine tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
21685-1-AP targets Trehalase in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag16246 Product name: Recombinant human TREH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 203-552 aa of BC109206 Sequence: YLTHTNDTAFLQENIETLALELDFWTKNRTVSVSLEGKNYLLNRYYVPYGGPRPESYSKDVELADTLPEGDREALWAELKAGAESGWDFSSRWLIGGPNPNSLSGIRTSKLVPVDLNAFLCQAEELMSNFYSRLGNDSQATKYRILRSQRLAALNTVLWDEQTGAWFDYDLEKKKKNREFYPSNLTPLWAGCFSDPGVADKALKYLEDNRILTYQYGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRTNFDVYSQKSAMYEKYDVSNGGQPGGGGEYEVQEGFGWTNGVVLMLLDRYGDRLTSGAKLAFLEPHCLAATLLPSLLLSLLPW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | trehalase (brush-border membrane glycoprotein) |
| 别名 | TREH, TREA, TRE, EC:3.2.1.28, Alpha,alpha-trehalose glucohydrolase |
| 计算分子量 | 583 aa, 67 kDa |
| 观测分子量 | 67-70 kDa |
| GenBank蛋白编号 | BC109206 |
| 基因名称 | Trehalase |
| Gene ID (NCBI) | 11181 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | O43280 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Trehalase is the enzyme hydrolyzing trehalose and belongs to the glycosyl hydrolase 37 family. In humans and most mammals, trehalase is found in the brush borders of the intestinal mucosa, kidney, liver, and blood plasma. Trehalases are conserved enzymes that catalyze the hydrolysis of one of two glycoside bonds of trehalose. With the exception of vertebrates where trehalase is required for the degradation of ingested trehalose, in other animal groups it is involved in diverse physiological processes including carbohydrate metabolism, resumption of growth in certain resting cells, germination of spores in fungi, recovery from stress conditions, etc (PMID: 25429048, PMID: 30628830).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Trehalase antibody 21685-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |