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验证数据展示

  • IHC staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    IHC staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Titin antibody included
  • IF Staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    IF Staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    IHC staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Titin antibody included
  • IHC staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    IHC staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Titin antibody included
  • IF Staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    IF Staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    IF Staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    IF Staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 27867-1-AP (same clone as 27867-1-PBS)

    IF Staining of NIH/3T3 using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Titin antibody (27867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

Titin Polyclonal antibody, PBS Only

Titin Polyclonal Antibody for IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 27867-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, IF-P, Indirect ELISA

别名

TTN

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Titin Ready-To-Use IHC Kit. Titin primary antibody included.

产品信息

27867-1-PBS targets Titin in IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27496

Product name: Recombinant human TTN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 33738-33969 aa of NM_001256850

Sequence: MFKSIHEKVSKISETKKSDQKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSVSSSCKLTIKAIKDTEAQKVSTQKTSEITPQKKAVVQEEISQKALRSEEIKMSEAKSQEKLALKEEASKVLISEEVKKSAATSLEKSIVHEEITKTSQASEEVRTHAEIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 titin
别名 TTN
计算分子量 3816 kDa
GenBank蛋白编号NM_001256850
基因名称 Titin
Gene ID (NCBI) 7273
RRIDAB_2880998
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WZ42
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Titin, or connectin, is a giant muscle protein expressed in the cardiac and skeletal muscles that spans half of the sarcomere from Z line to M line. It plays a key role in muscle contraction and relaxation, maintaining the structural integrity of the myotome(PMID: 11846417). Mutations in Titin are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma(PMID: 15802564).

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