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CoraLite® Plus 488-conjugated Tissue factor Monoclonal antibody

Tissue factor Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E4A1

Cat No : CL488-67056

Print datasheet

Synonyms

CD142, Coagulation factor III, F3, TF, TFA, Thromboplastin, Tissue factor

验证数据展示

  • FC experiment of A431 using CL488-67056

    FC experiment of A431 using CL488-67056

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Tissue factor (CL488-67056, Clone: 2E4A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67056-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67056 targets Tissue factor in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen Tissue factor fusion protein Ag27563 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coagulation factor III (thromboplastin, tissue factor)
Synonyms CD142, Coagulation factor III, F3, TF, TFA, Thromboplastin, Tissue factor
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC011029
Gene Symbol F3
Gene ID (NCBI) 2152
RRIDAB_2919414
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13726
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Tissue factor antibody CL488-67056Download protocol
Standard Protocols
Click here to view our Standard Protocols