• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Tissue Factor Monoclonal antibody

Tissue factor Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

2E4A1

Cat No : 67056-1-Ig

Print datasheet

Synonyms

CD142, Coagulation factor III, F3, TF, TFA, Thromboplastin, Tissue factor

验证数据展示

  • WB analysis of A431 using 67056-1-Ig

    WB analysis of A431 using 67056-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67056-1-Ig (Tissue factor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HUVEC using 67056-1-Ig

    FC experiment of HUVEC using 67056-1-Ig

    1X10^6 HUVEC cells were intracellularly stained with 0.2 ug Anti-Human Tissue Factor (67056-1-Ig, Clone:2E4A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, human blood tissue
Positive FC detected inHUVEC cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67056-1-Ig targets Tissue factor in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, WB,ELISA
Tested Reactivity Human
Immunogen Tissue factor fusion protein Ag27563 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coagulation factor III (thromboplastin, tissue factor)
Synonyms CD142, Coagulation factor III, F3, TF, TFA, Thromboplastin, Tissue factor
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC011029
Gene Symbol F3
Gene ID (NCBI) 2152
RRIDAB_2882367
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13726
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Tissue factor antibody 67056-1-IgDownload protocol
FC protocol for Tissue factor antibody 67056-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols