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验证数据展示

  • WB analysis of human placenta using 83627-1-RR (same clone as 83627-1-PBS)

    WB analysis of human placenta using 83627-1-RR (same clone as 83627-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83627-1-RR (Timp-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83627-1-RR (same clone as 83627-1-PBS)

    IHC staining of human placenta using 83627-1-RR (same clone as 83627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83627-1-RR (Timp-3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83627-1-RR (same clone as 83627-1-PBS)

    IHC staining of human tonsillitis using 83627-1-RR (same clone as 83627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83627-1-RR (Timp-3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

  • FC experiment of MDA-MB-231 using 83627-1-RR (same clone as 83627-1-PBS)

    FC experiment of MDA-MB-231 using 83627-1-RR (same clone as 83627-1-PBS)

    1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug Timp-3 Recombinant antibody (83627-1-RR, Clone:240374D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83627-1-RR (same clone as 83627-1-PBS)

    FC experiment of HeLa using 83627-1-RR (same clone as 83627-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Timp-3 Recombinant antibody (83627-1-RR, Clone:240374D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83627-1-RR

    Affinity and Kinetic Characterization of 83627-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83627-1-RR against Human Timp-3 were performed. The affinity constant is 2.63 nM.

Timp-3 Recombinant monoclonal antibody, PBS Only

Timp-3 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83627-1-PBS

产品说明书

CloneNo. 240374D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

TIMP3, 240374D8, HSMRK222, K222, K222TA2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83627-1-PBS targets Timp-3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34657

Product name: Recombinant human Timp-3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 168-211 aa of BC014277

Sequence: MLSNFGYPGYQSKHYACIRQKGGYCSWYRGWAPPDKSIINATDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TIMP metallopeptidase inhibitor 3
别名 TIMP3, 240374D8, HSMRK222, K222, K222TA2
计算分子量 24 kDa
观测分子量 24-33 kDa
GenBank蛋白编号BC014277
基因名称 TIMP3
Gene ID (NCBI) 7078
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP35625
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Timp-3 (TIMP metallopeptidase inhibitor 3), also known as SFD. It is expected to be located in extracellular space, and the protein is enriched in the placenta tissue and fat tissue. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation, and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. TIMP-3 is expressed as an unglycosylated 24 kDa and glycosylated 29 kDa protein with inhibitory activity against interstitial collagenase, stromelysin-1, and gelatinases A and B (PMID:11827795).

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