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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Thymidylate synthase Polyclonal antibody

Thymidylate synthase Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-15047

Print datasheet

Synonyms

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS

验证数据展示

  • FC experiment of HeLa using CL647-15047

    FC experiment of HeLa using CL647-15047

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Thymidylate synthase (CL647-15047) (red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL647-15047

    IF Staining of HeLa using CL647-15047

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 Thymidylate synthase antibody (CL647-15047) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TYMS Ready-To-Use IHC Kit. TYMS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15047-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-15047 targets Thymidylate synthase in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen Thymidylate synthase fusion protein Ag7027 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thymidylate synthetase
Synonyms HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC002567
Gene Symbol TYMS
Gene ID (NCBI) 7298
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04818
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Thymidylate synthase antibody CL647-15047Download protocol
Standard Protocols
Click here to view our Standard Protocols