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验证数据展示

  • WB analysis using 86013-3-RR

    WB analysis using 86013-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86013-3-RR (Testin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 86013-3-RR

    IP experiment of K-562 using 86013-3-RR

    IP result of anti-Testin (IP:86013-3-RR, 4ug; Detection:86013-3-RR 1:1000) with K-562 cells lysate 2440 ug.

  • IF Staining of K-562 using 86013-3-RR

    IF Staining of K-562 using 86013-3-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Testin antibody (86013-3-RR, Clone: 250432C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 86013-3-RR

    Affinity of 86013-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86013-3-RR against Human Testin were performed. The affinity constant is 2.17 nM.

Testin Recombinant monoclonal antibody

Testin Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 86013-3-RR

产品说明书

CloneNo. 250432C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

TES

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, mouse testis tissue, rat testis tissue, human testis tissue
Positive IP detected inK-562 cells
Positive IF/ICC detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86013-3-RR targets Testin in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0379

Product name: Recombinant human TES protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 108-323 aa of BC001451

Sequence: TVTYEWAPPVQNQALARQYMQMLPKEKQPVAGSEGAQYRKKQLAKQLPAHDQDPSKCHELSPREVKEMEQFVKKYKSEALGVGDVKLPCEMDAQGPKQMNIPGGDRSTPAAVGAMEDKSAEHKRTQYSCYCCKLSMKEGDPAIYAERAGYDKLWHPACFVCSTCHELLVDMIYFWKNEKLYCGRHYCDSEKPRCAGCDELIFSNEYTQAENQNWHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 testis derived transcript (3 LIM domains)
别名 TES
计算分子量 48 kDa
观测分子量 42-48 kDa
GenBank蛋白编号BC001451
基因名称 TES
Gene ID (NCBI) 26136
RRIDAB_3744550
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UGI8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Testin (TES) is a cytoskeleton-associated protein that localizes along actin stress fibers at cell-cell contact areas and at focal adhesion plaques. It interacts with a variety of cytoskeletal proteins and gets involved in regulation of cell adhesion and migration. TES is a putative tumor suppressor and reduced expression of TES has been reported in various cancers. In addition, TES is a marker for Sertoli-Germ Cell junction and is useful to monitor the disruption of intercellular junctions in the testis.

实验方案

Product Specific Protocols
IF protocol for Testin antibody 86013-3-RRDownload protocol
IP protocol for Testin antibody 86013-3-RRDownload protocol
WB protocol for Testin antibody 86013-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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