验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33326-1-AP targets Tesmin in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag39255 Product name: Recombinant human MTL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 158-306 aa of NM_004923.3 Sequence: VEIKEAGGTTTSNNPEEATLQNLLAQESCCKFPSSQELEDASCCSLKKDSNPMVICQLKGGTQMLCIDNSRTRELKALHLVPQYQDQNNYLQSDVPKPMTALVGRFLPASTKLNLITQQLEGALPSVVNGSAFPSGSTLPGPPKITLAG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | metallothionein-like 5, testis-specific (tesmin) |
| 别名 | Metallothionein-like 5, testis-specific, MTL5, Testis-specific metallothionein-like protein |
| 计算分子量 | 55kDa，508aa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | NM_004923.3 |
| 基因名称 | MTL5 |
| Gene ID (NCBI) | 9633 |
| RRID | AB_3742940 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Y4I5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Testis Expressed Metallothionein Like Protein (Tesmin), also known as Metallothionein-Like 5 (MTL5), encodes the metallothionein-like protein owning some characteristics of metallothionein. Tesmin is a 60 kDa protein which has cysteine-rich motifs (CXC domain), characteristic of metallothioneins (MTs). Tesmin was closely related to meiosis in the reproductive process. As a testis-specific protein, the localization of Tesmin changed dynamically with the progression of meiosis in a mouse experiment, the process of which might be responsive to metal stress like cadmium by oxidative stress (Cd), as well as require the involvement of LIN9. So far, the expression of Tesmin in adult humans has been observed in prostate and gastric cancer (PMID: 39229988, PMID: 33281959, PMID: 31059101).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Tesmin antibody 33326-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
