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CoraLite® Plus 488-conjugated TWIST2 Monoclonal antibody

TWIST2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B12D11

Cat No : CL488-66544

Print datasheet

Synonyms

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

验证数据展示

  • IF Staining of HepG2 using CL488-66544

    IF Staining of HepG2 using CL488-66544

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated TWIST2 antibody (CL488-66544, Clone: 1B12D11 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TWIST2 Ready-To-Use IHC Kit. TWIST2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66544-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66544 targets TWIST2 in IF applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IF/ICC
Tested Reactivity Human, rat, mouse
Immunogen TWIST2 fusion protein Ag2348 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name twist homolog 2 (Drosophila)
Synonyms bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2
Calculated Molecular Weight 160 aa, 18 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC033168
Gene Symbol TWIST2
Gene ID (NCBI) 117581
RRIDAB_2919341
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WVJ9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TWIST2 antibody CL488-66544Download protocol
Standard Protocols
Click here to view our Standard Protocols