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验证数据展示

  • WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

    WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

    IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 67802-1-Ig (same clone as 67802-1-PBS)

    IP experiment of HepG2 using 67802-1-Ig (same clone as 67802-1-PBS)

    IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67802-1-Ig (same clone as 67802-1-PBS)

    IF Staining of mouse brain using 67802-1-Ig (same clone as 67802-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TUFM antibody (67802-1-Ig, Clone: 2A10D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67802-1-Ig (same clone as 67802-1-PBS)

    IF Staining of mouse brain using 67802-1-Ig (same clone as 67802-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TUFM antibody (67802-1-Ig, Clone: 2A10D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

TUFM Monoclonal antibody, PBS Only

TUFM Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 67802-1-PBS

产品说明书

CloneNo. 2A10D2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

2A10D2, COXPD4, EF Tu, EF TuMT, EFTU

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67802-1-PBS targets TUFM in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag24976

Product name: Recombinant human TUFM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 108-455 aa of BC010041

Sequence: GITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Tu translation elongation factor, mitochondrial
别名 2A10D2, COXPD4, EF Tu, EF TuMT, EFTU
计算分子量 50 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC010041
基因名称 TUFM
Gene ID (NCBI) 7284
RRIDAB_2918565
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49411
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

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