验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HCT 116 cells, HepG2 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, 4T1 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67802-1-Ig targets TUFM in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag24976 Product name: Recombinant human TUFM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 108-455 aa of BC010041 Sequence: GITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Tu translation elongation factor, mitochondrial |
| 别名 | EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC010041 |
| 基因名称 | TUFM |
| Gene ID (NCBI) | 7284 |
| RRID | AB_2918565 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49411 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TUFM antibody 67802-1-Ig | Download protocol |
| IP protocol for TUFM antibody 67802-1-Ig | Download protocol |
| WB protocol for TUFM antibody 67802-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
