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验证数据展示

  • WB analysis using 67802-1-Ig

    WB analysis using 67802-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67802-1-Ig

    IHC staining of human colon cancer using 67802-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HepG2 using 67802-1-Ig

    IP experiment of HepG2 using 67802-1-Ig

    IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug.

TUFM Monoclonal antibody

TUFM Monoclonal Antibody for WB, IHC, IP, ELISA
Cat No. 67802-1-Ig

产品说明书

CloneNo. 2A10D2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HCT 116 cells, HepG2 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, 4T1 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67802-1-Ig targets TUFM in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TUFM fusion protein Ag24976 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Tu translation elongation factor, mitochondrial
别名 EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4
计算分子量 50 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC010041
基因名称 TUFM
Gene ID (NCBI) 7284
RRIDAB_2918565
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49411
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

实验方案

Product Specific Protocols
WB protocol for TUFM antibody 67802-1-IgDownload protocol
IHC protocol for TUFM antibody 67802-1-IgDownload protocol
IP protocol for TUFM antibody 67802-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols