×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 67802-1-Ig

    WB analysis using 67802-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67802-1-Ig

    IHC staining of human colon cancer using 67802-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HepG2 using 67802-1-Ig

    IP experiment of HepG2 using 67802-1-Ig

    IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug.

  • IF Staining of mouse brain using 67802-1-Ig

    IF Staining of mouse brain using 67802-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TUFM antibody (67802-1-Ig, Clone: 2A10D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 67802-1-Ig

    IF Staining of mouse brain using 67802-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TUFM antibody (67802-1-Ig, Clone: 2A10D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TUFM Monoclonal antibody

TUFM Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 67802-1-Ig

产品说明书

CloneNo. 2A10D2
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA

别名

2A10D2, COXPD4, EF Tu, EF TuMT, EFTU

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inLNCaP cells, HCT 116 cells, HepG2 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, 4T1 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67802-1-Ig targets TUFM in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse, rat
免疫原

CatNo: Ag24976

Product name: Recombinant human TUFM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 108-455 aa of BC010041

Sequence: GITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Tu translation elongation factor, mitochondrial
别名 2A10D2, COXPD4, EF Tu, EF TuMT, EFTU
计算分子量 50 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC010041
基因名称 TUFM
Gene ID (NCBI) 7284
RRIDAB_2918565
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49411
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

实验方案

Product Specific Protocols
IF protocol for TUFM antibody 67802-1-IgDownload protocol
IHC protocol for TUFM antibody 67802-1-IgDownload protocol
IP protocol for TUFM antibody 67802-1-IgDownload protocol
WB protocol for TUFM antibody 67802-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

Decoding CKD-induced muscle atrophy through the critical role of lncRNA GAS5 and pyroptosis

Authors - Rong Huang
View Article
ratWB

Ecotoxicol Environ Saf

Mitochondrial translation impairment-triggered neuroinflammation mediates fluoride-induced cognitive deficits.

Authors - Wenhui Liu
View Article
||
New chat

Able™

正在加载，请稍候...