Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IP, IF/ICC, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IF/ICC, FC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11224-1-AP

Print datasheet

Synonyms

alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, TUBA1B, tubulin, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, tubulin, alpha 1b, α-tubulin



经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, rat brain
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells, MCF-7 cells, Lymphatic endothelial cells cells
Positive FC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11224-1-AP targets Alpha Tubulin in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF/ICC, FC, IHC, ELISA
Cited ApplicationsWB, IP, IF, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, monkey, rabbit, zebrafish, P. lividus, hamster
Immunogen Alpha Tubulin fusion protein Ag1727 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, alpha 1b
Synonyms alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, TUBA1B, tubulin, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, tubulin, alpha 1b, α-tubulin
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC009314
Gene Symbol TUBA1B
Gene ID (NCBI) 10376
RRIDAB_2210206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68363
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).


实验方案

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IF protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IP protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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humanIF

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Authors - Aleksandra Klimas
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Nature

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Mol Cancer

A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

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Nat Med

Therapeutic efficacy of antisense oligonucleotides in mouse models of CLN3 Batten disease.

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Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

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