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验证数据展示

  • WB analysis using 21447-1-AP (same clone as 21447-1-PBS)

    WB analysis using 21447-1-AP (same clone as 21447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21447-1-AP (TTLL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21447-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 21447-1-AP (same clone as 21447-1-PBS)

    IHC staining of mouse testis using 21447-1-AP (same clone as 21447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21447-1-AP (TTLL12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21447-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TTLL12 antibody included
  • IF Staining of mouse testis using 21447-1-AP (same clone as 21447-1-PBS)

    IF Staining of mouse testis using 21447-1-AP (same clone as 21447-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TTLL12 antibody (21447-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21447-1-PBS in a different storage buffer formulation.

TTLL12 Polyclonal antibody, PBS Only

TTLL12 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 21447-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

Inactive tubulin--tyrosine ligase-like protein 12, Tubulin--tyrosine ligase-like protein 12

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TTLL12 Ready-To-Use IHC Kit. TTLL12 primary antibody included.

产品信息

21447-1-PBS targets TTLL12 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13804

Product name: Recombinant human TTLL12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 293-644 aa of BC001070

Sequence: DINPVVHPHGHIFKVYTDVQQVASSLTHPRFTLTQSEADADILFNFSHFKDYRKLSQERPGVLLNQFPCENLLTVKDCLASIARRAGGPEGPPWLPRTFNLRTELPQFVSYFQQRERWGEDNHWICKPWNLARSLDTHVTKSLHSIIRHRESTPKVVSKYIESPVLFLREDVGKVKFDIRYIVLLRSVRPLRLFVYDVFWLRFSNRAFALNDLDDYEKHFTVMNYDPDVVLKQVHCEEFIPEFEKQYPEFPWTDVQAEIFRAFTELFQVACAKPPPLGLCDYPSSRAMYAVDLMLKWDNGPDGRRVMQPQILEVNFNPDCERACRYHPTFFNDVFSTLFLDQPGGCHVTCLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin tyrosine ligase-like family, member 12
别名 Inactive tubulin--tyrosine ligase-like protein 12, Tubulin--tyrosine ligase-like protein 12
计算分子量 644 aa, 74 kDa
观测分子量 75 kDa
GenBank蛋白编号BC001070
基因名称 TTLL12
Gene ID (NCBI) 23170
RRIDAB_3669368
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14166
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).

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