验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, LNCaP cells |
Planning an IHC experiment? We recommend our IHCeasy TTLL12 Ready-To-Use IHC Kit. TTLL12 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
21447-1-AP targets TTLL12 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TTLL12 fusion protein Ag13804 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tubulin tyrosine ligase-like family, member 12 |
| 别名 | Tubulin--tyrosine ligase-like protein 12, Inactive tubulin--tyrosine ligase-like protein 12 |
| 计算分子量 | 644 aa, 74 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC001070 |
| 基因名称 | TTLL12 |
| Gene ID (NCBI) | 23170 |
| RRID | AB_3669368 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14166 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TTLL12 antibody 21447-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |