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验证数据展示

  • WB analysis using 21447-1-AP

    WB analysis using 21447-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21447-1-AP (TTLL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 21447-1-AP

    IHC staining of mouse testis using 21447-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21447-1-AP (TTLL12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTLL12 antibody included

TTLL12 Polyclonal antibody

TTLL12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21447-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Inactive tubulin--tyrosine ligase-like protein 12, Tubulin--tyrosine ligase-like protein 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TTLL12 Ready-To-Use IHC Kit. TTLL12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

21447-1-AP targets TTLL12 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 TTLL12 fusion protein Ag13804 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin tyrosine ligase-like family, member 12
别名 Inactive tubulin--tyrosine ligase-like protein 12, Tubulin--tyrosine ligase-like protein 12
计算分子量 644 aa, 74 kDa
观测分子量 75 kDa
GenBank蛋白编号BC001070
基因名称 TTLL12
Gene ID (NCBI) 23170
RRIDAB_3669368
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14166
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).

实验方案

Product Specific Protocols
WB protocol for TTLL12 antibody 21447-1-APDownload protocol
IHC protocol for TTLL12 antibody 21447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Int J Clin Exp Pathol

TTLL12 expression in ovarian cancer correlates with a poor outcome.

Authors - Shangjie Yang
View Article