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验证数据展示

  • IHC staining of mouse brain using 13618-1-AP

    IHC staining of mouse brain using 13618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTL antibody included
  • IF Staining of mouse brain using 13618-1-AP

    IF Staining of mouse brain using 13618-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13618-1-AP (TTL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 13618-1-AP

    IHC staining of mouse brain using 13618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTL antibody included
  • WB analysis of HeLa using 13618-1-AP

    WB analysis of HeLa using 13618-1-AP

    WB result of TTL antibody (13618-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTL transfected HeLa cells.

  • WB analysis using 13618-1-AP

    WB analysis using 13618-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13618-1-AP (TTL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13618-1-AP

    IHC staining of human placenta using 13618-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTL antibody included
  • IHC staining of mouse lung using 13618-1-AP

    IHC staining of mouse lung using 13618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTL antibody included
  • IHC staining of mouse skeletal muscle using 13618-1-AP

    IHC staining of mouse skeletal muscle using 13618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTL antibody included
  • IHC staining of rat lung using 13618-1-AP

    IHC staining of rat lung using 13618-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTL antibody included
  • IHC staining of rat skeletal muscle using 13618-1-AP

    IHC staining of rat skeletal muscle using 13618-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTL antibody included
  • IHC staining of human placenta using 13618-1-AP

    IHC staining of human placenta using 13618-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTL antibody included
  • Featured Product
  • KD/KO Validated

TTL Polyclonal antibody

TTL Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 13618-1-AP

产品说明书

引用文献(35)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF-P, ELISA and More (1)

别名

EC:6.3.2.25, Tubulin tyrosine ligase, tubulin tyrosine ligase, Tubulin--tyrosine ligase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain, rat brain
Positive IHC detected inmouse brain tissue, rat lung tissue, human placenta tissue, mouse lung tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy® TTL Ready-To-Use IHC Kit. TTL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13618-1-AP targets TTL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine
免疫原

CatNo: Ag4526

Product name: Recombinant human TTL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-377 aa of BC036819

Sequence: MYTFVVRDENSSVYAEVSRLLLATGHWKRLRRDNPRFNLMLGERNRLPFGRLGHEPGLVQLVNYYRGADKLCRKASLVKLIKTSPELAESCTWFPESYVIYPTNLKTPVAPAQNGIQPPISNSRTDEREFFLASYNRKKEDGEGNVWIAKSSAGAKGEGILISSEASELLDFIDNQGQVHVIQKYLEHPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFKEFNQYLTSALNITLESSILLQIKHIIRNCLLSVEPAISTKHLPYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDIAISSVFPPPDVEQPQTQPAAFIKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin tyrosine ligase
别名 EC:6.3.2.25, Tubulin tyrosine ligase, tubulin tyrosine ligase, Tubulin--tyrosine ligase
计算分子量 377 aa, 43 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC036819
基因名称 TTL
Gene ID (NCBI) 150465
RRIDAB_2256858
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NG68
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

实验方案

Product Specific Protocols
IF protocol for TTL antibody 13618-1-APDownload protocol
IHC protocol for TTL antibody 13618-1-APDownload protocol
WB protocol for TTL antibody 13618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
View Article
mouseWB

Nature

MARK4 controls ischaemic heart failure through microtubule detyrosination.

Authors - Xian Yu
View Article
humanWB

Nat Med

Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.

Authors - Christina Yingxian Chen
View Article
humanWB

Science

Mitosis. Microtubule detyrosination guides chromosomes during mitosis.

Authors - Marin Barisic
View Article
humanWB

Science

Vasohibins encode tubulin detyrosinating activity.

Authors - Joppe Nieuwenhuis
View Article
WB

Science

Posttranslational modification of microtubules by the MATCAP detyrosinase.

Authors - Lisa Landskron
View Article
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