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TTL Polyclonal antibody

TTL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13618-1-AP

Print datasheet

Synonyms

TTL, Tubulin tyrosine ligase, tubulin tyrosine ligase



经过测试的应用

Positive WB detected inHeLa cells, mouse brain, rat brain
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13618-1-AP targets TTL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Immunogen TTL fusion protein Ag4526 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin tyrosine ligase
Synonyms TTL, Tubulin tyrosine ligase, tubulin tyrosine ligase
Calculated Molecular Weight 377 aa, 43 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC036819
Gene Symbol TTL
Gene ID (NCBI) 150465
RRIDAB_2256858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG68
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

实验方案

Product Specific Protocols
WB protocol for TTL antibody 13618-1-APDownload protocol
IHC protocol for TTL antibody 13618-1-APDownload protocol
IF protocol for TTL antibody 13618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Science

Posttranslational modification of microtubules by the MATCAP detyrosinase.

Authors - Lisa Landskron
mouseWB

Nature

MARK4 controls ischaemic heart failure through microtubule detyrosination.

Authors - Xian Yu
humanWB

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
humanWB

Science

Vasohibins encode tubulin detyrosinating activity.

Authors - Joppe Nieuwenhuis
humanWB

Science

Mitosis. Microtubule detyrosination guides chromosomes during mitosis.

Authors - Marin Barisic
humanWB

Nat Med

Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.

Authors - Christina Yingxian Chen