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TTL Polyclonal antibody

TTL Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13618-1-AP

Print datasheet

Synonyms

TTL, Tubulin tyrosine ligase, tubulin tyrosine ligase



经过测试的应用

Positive WB detected inHeLa cells, mouse brain, rat brain
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13618-1-AP targets TTL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Immunogen TTL fusion protein Ag4526 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin tyrosine ligase
Synonyms TTL, Tubulin tyrosine ligase, tubulin tyrosine ligase
Calculated Molecular Weight 377 aa, 43 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC036819
Gene Symbol TTL
Gene ID (NCBI) 150465
RRIDAB_2256858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG68
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

实验方案

Product Specific Protocols
WB protocol for TTL antibody 13618-1-APDownload protocol
IHC protocol for TTL antibody 13618-1-APDownload protocol
IF protocol for TTL antibody 13618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Science

Posttranslational modification of microtubules by the MATCAP detyrosinase.

Authors - Lisa Landskron
mouseWB

Nature

MARK4 controls ischaemic heart failure through microtubule detyrosination.

Authors - Xian Yu
humanWB

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
humanWB

Science

Vasohibins encode tubulin detyrosinating activity.

Authors - Joppe Nieuwenhuis
humanWB

Science

Mitosis. Microtubule detyrosination guides chromosomes during mitosis.

Authors - Marin Barisic
humanWB

Nat Med

Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.

Authors - Christina Yingxian Chen