TTF2 Polyclonal antibody
TTF2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | A2780 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
Planning an IHC experiment? We recommend our IHCeasy TTF2 Ready-To-Use IHC Kit. TTF2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
13722-1-AP targets TTF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Immunogen | TTF2 fusion protein Ag4671 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transcription termination factor, RNA polymerase II |
| Synonyms | F2, HuF2, Lodestar homolog, Transcription release factor 2, TTF2 |
| Calculated Molecular Weight | 1162 aa, 129 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC030058 |
| Gene Symbol | TTF2 |
| Gene ID (NCBI) | 8458 |
| RRID | AB_2262865 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transcription elongation is an key target for control of eukaryotic gene expression. After initiating from a promoter, the RNA polymerase II elongation complex is subjected to a block to elongation by the action of components of N-TEF (negative transcription elongation factor) [PMID: 9748214]. Transcription termination factor 2 (TTF2) is a DsDNA-dependent ATPase that acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. It also contribute to mitotic transcription repression, and involved in pre-mRNA splicing[PMID:15125840, 12927788].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TTF2 antibody 13722-1-AP | Download protocol |
| IHC protocol for TTF2 antibody 13722-1-AP | Download protocol |
| IF protocol for TTF2 antibody 13722-1-AP | Download protocol |
| IP protocol for TTF2 antibody 13722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell mRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription. | ||
Sci Total Environ PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism. | ||
Nat Commun Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features |
