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验证数据展示

  • WB analysis of NIH/3T3 using 25410-1-AP (same clone as 25410-1-PBS)

    WB analysis of NIH/3T3 using 25410-1-AP (same clone as 25410-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25410-1-AP (TTDN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25410-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 25410-1-AP (same clone as 25410-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 25410-1-AP (same clone as 25410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25410-1-AP (TTDN1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25410-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MPLKIP/TTDN1 antibody included

TTDN1 Polyclonal antibody, PBS Only

TTDN1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25410-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

C7orf11, ABHS, TTD non-photosensitive 1 protein, ORF20, MPLKIP

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MPLKIP/TTDN1 Ready-To-Use IHC Kit. MPLKIP/TTDN1 primary antibody included.

产品信息

25410-1-PBS targets TTDN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22094

Product name: Recombinant human C7orf11 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-179 aa of BC026265

Sequence: MQRQNFRPPTPPYPGPGGGGWGSGSSFRGTPGGGGPRPPSPRDGYGSPHHTPPYGPRSRPYGSSHSPRHGGSFPGGRFGSPSPGGYPGSYSRSPAGSQQQFGYSPGQQQTHPQGSPRTSTPFGSGRVREKRMSNELENYFKPSMLEDPWAGLEPVSVVDISQQYSNTQTFTGKKGRYFC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 11
别名 C7orf11, ABHS, TTD non-photosensitive 1 protein, ORF20, MPLKIP
计算分子量 179 aa, 19 kDa
观测分子量23 kDa
GenBank蛋白编号BC026265
基因名称 TTDN1
Gene ID (NCBI) 136647
RRIDAB_2935461
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAP9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TTDN1 colocalizes with Plk1 at the centrosome in mitosis and the midbody during cytokinesis. It is phosphorylated by Cdk1 in mitosis, and this is required for its interaction with Plk1. Overexpression of TTDN1 or its knockdown by siRNA causes multi-polar spindles and multiple nuclei, suggesting that TTDN1 plays a role in regulating mitosis and cytokinesis. Mutations in the TTDN1 gene are associated with a distinct trichothiodystrophy phenotype. (PMID:17310276, 25290684)

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