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验证数据展示

  • WB analysis of NIH/3T3 using 25410-1-AP

    WB analysis of NIH/3T3 using 25410-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25410-1-AP (TTDN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 25410-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 25410-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25410-1-AP (TTDN1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MPLKIP/TTDN1 antibody included

TTDN1 Polyclonal antibody

TTDN1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25410-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C7orf11, ABHS, TTD non-photosensitive 1 protein, ORF20, MPLKIP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MPLKIP/TTDN1 Ready-To-Use IHC Kit. MPLKIP/TTDN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25410-1-AP targets TTDN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TTDN1 fusion protein Ag22094 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 11
别名 C7orf11, ABHS, TTD non-photosensitive 1 protein, ORF20, MPLKIP
计算分子量 179 aa, 19 kDa
观测分子量23 kDa
GenBank蛋白编号BC026265
基因名称 TTDN1
Gene ID (NCBI) 136647
RRIDAB_2935461
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAP9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTDN1 colocalizes with Plk1 at the centrosome in mitosis and the midbody during cytokinesis. It is phosphorylated by Cdk1 in mitosis, and this is required for its interaction with Plk1. Overexpression of TTDN1 or its knockdown by siRNA causes multi-polar spindles and multiple nuclei, suggesting that TTDN1 plays a role in regulating mitosis and cytokinesis. Mutations in the TTDN1 gene are associated with a distinct trichothiodystrophy phenotype. (PMID:17310276, 25290684)

实验方案

Product Specific Protocols
WB protocol for TTDN1 antibody 25410-1-APDownload protocol
IHC protocol for TTDN1 antibody 25410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols