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验证数据展示

  • WB analysis using 84611-2-RR

    WB analysis using 84611-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84611-2-RR (TTC9C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84611-2-RR

    WB analysis using 84611-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84611-2-RR (TTC9C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 84611-2-RR

    WB analysis of HeLa using 84611-2-RR

    WB result of TTC9C antibody (84611-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC9C transfected HeLa cells.

  • IHC staining of mouse testis using 84611-2-RR

    IHC staining of mouse testis using 84611-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84611-2-RR (TTC9C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 84611-2-RR

    IF Staining of mouse testis using 84611-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TTC9C antibody (84611-2-RR, Clone: 242061D12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84611-2-RR

    Affinity and Kinetic Characterization of 84611-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84611-2-RR against Human TTC9C were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

TTC9C Recombinant monoclonal antibody

TTC9C Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84611-2-RR

产品说明书

CloneNo. 242061D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

242061D12, Tetratricopeptide repeat protein 9C, TPR repeat protein 9C

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells, HeLa cells, mouse thymus tissue, mouse skin tissue, mouse stomach tissue, human kidney tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84611-2-RR targets TTC9C in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag37081

Product name: Recombinant human TTC9C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-171 aa of BC032123

Sequence: MEKRLQEAQLYKEEGNQRYREGKYRDAVSRYHRALLQLRGLDPSLPSPLPNLGPQGPALTPEQENILHTTQTDCYNNLAACLLQMEPVNYERVREYSQKVLERQPDNAKALYRAGVAFFHLQDYDQARHYLLAAVNRQPKDANVRRYLQLTQSELSSYHRKEKQLYLGMFG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tetratricopeptide repeat domain 9C
别名 242061D12, Tetratricopeptide repeat protein 9C, TPR repeat protein 9C
观测分子量19-22 kDa
GenBank蛋白编号BC032123
基因名称 TTC9C
Gene ID (NCBI) 283237
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8N5M4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTC9 family belongs to tetratricopeptide repeat (TPR)-containing proteins family (PMID: 35949301).The TPR motifs are arranged in antiparallel α-helical hairpins which are clustered to form flexible grooved interfaces. Members in TRP-containing proteins family are involved in a diverse range of biological functions, including cell cycle control, transcription, protein folding, and steroid receptor signaling (PMID: 15497498;PMID: 10786835).

实验方案

Product Specific Protocols
IF protocol for TTC9C antibody 84611-2-RRDownload protocol
IHC protocol for TTC9C antibody 84611-2-RRDownload protocol
WB protocol for TTC9C antibody 84611-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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