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验证数据展示

  • WB analysis using 84611-2-RR (same clone as 84611-2-PBS)

    WB analysis using 84611-2-RR (same clone as 84611-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84611-2-RR (TTC9C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84611-2-PBS in a different storage buffer formulation.

  • WB analysis using 84611-2-RR (same clone as 84611-2-PBS)

    WB analysis using 84611-2-RR (same clone as 84611-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84611-2-RR (TTC9C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84611-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84611-2-RR (same clone as 84611-2-PBS)

    IHC staining of mouse testis using 84611-2-RR (same clone as 84611-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84611-2-RR (TTC9C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84611-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 84611-2-RR (same clone as 84611-2-PBS)

    IF Staining of mouse testis using 84611-2-RR (same clone as 84611-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TTC9C antibody (84611-2-RR, Clone: 242061D12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84611-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01448-1

    Cytometric bead array standard curve of MP01448-1

    Cytometric bead array standard curve of MP01448-1, TTC9C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84611-2-PBS. Detection antibody: 84611-1-PBS. Standard: Ag37081. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84611-2-RR

    Affinity and Kinetic Characterization of 84611-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84611-2-RR against Human TTC9C were performed. The affinity constant is below 1 pM.

TTC9C Recombinant antibody, PBS Only (Capture)

TTC9C Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 84611-2-PBS

产品说明书

CloneNo. 242061D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

242061D12, Tetratricopeptide repeat protein 9C, TPR repeat protein 9C

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84611-2-PBS targets TTC9C as part of a matched antibody pair:

MP01448-1: 84611-2-PBS capture and 84611-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TTC9C fusion protein Ag37081 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tetratricopeptide repeat domain 9C
别名 242061D12, Tetratricopeptide repeat protein 9C, TPR repeat protein 9C
观测分子量19-22 kDa
GenBank蛋白编号BC032123
基因名称 TTC9C
Gene ID (NCBI) 283237
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N5M4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TTC9 family belongs to tetratricopeptide repeat (TPR)-containing proteins family (PMID: 35949301).The TPR motifs are arranged in antiparallel α-helical hairpins which are clustered to form flexible grooved interfaces. Members in TRP-containing proteins family are involved in a diverse range of biological functions, including cell cycle control, transcription, protein folding, and steroid receptor signaling (PMID: 15497498;PMID: 10786835).