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TTC39A Polyclonal antibody

TTC39A Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21323-1-AP

Print datasheet

Synonyms

C1orf34, DEME 6, KIAA0452, TPR repeat protein 39A, TTC39A

验证数据展示

  • WB analysis of mouse liver using 21323-1-AP

    WB analysis of mouse liver using 21323-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21323-1-AP (TTC39A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 21323-1-AP

    IHC staining of human breast cancer using 21323-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 21323-1-AP

    IHC staining of human breast cancer using 21323-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21323-1-AP

    IF Staining of HepG2 using 21323-1-AP

    Immunofluorescent analysis of HepG2 cells, using TTC39A antibody 21323-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inmouse liver tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21323-1-AP is TTC39A Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen TTC39A fusion protein Ag14874 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 39A
Synonyms C1orf34, DEME 6, KIAA0452, TPR repeat protein 39A, TTC39A
Calculated Molecular Weight 613 aa, 70 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC004399
Gene Symbol TTC39A
Gene ID (NCBI) 22996
RRIDAB_10733645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SRH9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TTC39A antibody 21323-1-APDownload protocol
IHC protocol for TTC39A antibody 21323-1-APDownload protocol
IF protocol for TTC39A antibody 21323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols