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验证数据展示

  • WB analysis of mouse liver using 21323-1-AP

    WB analysis of mouse liver using 21323-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21323-1-AP (TTC39A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 21323-1-AP

    IHC staining of human breast cancer using 21323-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 21323-1-AP

    IHC staining of human breast cancer using 21323-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21323-1-AP

    IF Staining of HepG2 using 21323-1-AP

    Immunofluorescent analysis of HepG2 cells, using TTC39A antibody 21323-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

TTC39A Polyclonal antibody

TTC39A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21323-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

C1orf34, DEME 6, KIAA0452, TPR repeat protein 39A, TTC39A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21323-1-AP targets TTC39A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14874

Product name: Recombinant human TTC39A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 122-221 aa of BC004399

Sequence: CLKYLGRVQEAEENFRSISANEKKIKYDHYLIPNALLELALLLMEQDRNEEAIKLLESAKQNYKNYSMESRTHFRIQAATLQAKSSLENSSRSMVSSVSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 39A
别名 C1orf34, DEME 6, KIAA0452, TPR repeat protein 39A, TTC39A
计算分子量 613 aa, 70 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC004399
基因名称 TTC39A
Gene ID (NCBI) 22996
RRIDAB_10733645
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5SRH9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TTC39A antibody 21323-1-APDownload protocol
IHC protocol for TTC39A antibody 21323-1-APDownload protocol
WB protocol for TTC39A antibody 21323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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