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验证数据展示

  • WB analysis using 68232-1-Ig (same clone as 68232-1-PBS)

    WB analysis using 68232-1-Ig (same clone as 68232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68232-1-PBS in a different storage buffer formulation.

  • WB analysis using 68232-1-Ig (same clone as 68232-1-PBS)

    WB analysis using 68232-1-Ig (same clone as 68232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68232-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 68232-1-Ig (same clone as 68232-1-PBS)

    IF Staining of hTERT-RPE1 using 68232-1-Ig (same clone as 68232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC30A antibody (68232-1-Ig, Clone: 1A11B3 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, green). This data was developed using the same antibody clone with 68232-1-PBS in a different storage buffer formulation.

TTC30A Monoclonal antibody, PBS Only

TTC30A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68232-1-PBS

产品说明书

CloneNo. 1A11B3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, Indirect ELISA

别名

FLJ13946, FLJ77601, TPR repeat protein 30A, TTC30A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68232-1-PBS targets TTC30A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag18361

Product name: Recombinant human TTC30A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-342 aa of BC042848

Sequence: MAGLSGAQIPDGEFTALVYRLIRDARYAEAVQLLGRELQRSPRSRAGLSLLGYCYYRLQEFALAAECYEQLGQLHPELEQYRLYQAQALYKACLYPEATRVAFLLLDNPAYHSRVLRLQAAIKYSEGDLPGSRSLVEQLLSGEGGEESGGDNETDGQVNLGCLLYKEGQYEAACSKFSATLQASGYQPDLSYNLALAYYSSRQYASALKHIAEIIERGIRQHPELGVGMTTEGFDVRSVGNTLVLHQTALVEAFNLKAAIEYQLRNYEVAQETLTDMPPRAEEELDPVTLHNQALMNMDARPTEGFEKLQFLLQQNPFPPETFGNLLLLYCKYEYFDLAADV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 30A
别名 FLJ13946, FLJ77601, TPR repeat protein 30A, TTC30A
计算分子量 665 aa, 76 kDa
观测分子量 72-76 kDa
GenBank蛋白编号BC042848
基因名称 TTC30A
Gene ID (NCBI) 92104
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ86WT1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tetratricopeptide repeat protein 30A (TTC30A) as a component of ciliary segmentation, may be essential for cartilage differentiation and renal tubulogenesis (PMID: 34548398). It is required for polyglutamylation of axonemal tubulin and plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.

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