验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, rat testis tissue, HeLa cells, HEK-293 cells, mouse testis tissue |
Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68232-1-Ig targets TTC30A in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human, Mouse, Rat |
免疫原 | TTC30A fusion protein Ag18361 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | tetratricopeptide repeat domain 30A |
别名 | FLJ13946, FLJ77601, TPR repeat protein 30A, TTC30A |
计算分子量 | 665 aa, 76 kDa |
观测分子量 | 72-76 kDa |
GenBank蛋白编号 | BC042848 |
基因名称 | TTC30A |
Gene ID (NCBI) | 92104 |
RRID | AB_2935319 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q86WT1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tetratricopeptide repeat protein 30A (TTC30A) as a component of ciliary segmentation, may be essential for cartilage differentiation and renal tubulogenesis (PMID: 34548398). It is required for polyglutamylation of axonemal tubulin and plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.
实验方案
Product Specific Protocols | |
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WB protocol for TTC30A antibody 68232-1-Ig | Download protocol |
IF protocol for TTC30A antibody 68232-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |