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验证数据展示

  • WB analysis using 68232-1-Ig

    WB analysis using 68232-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68232-1-Ig

    WB analysis using 68232-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 68232-1-Ig

    IF Staining of hTERT-RPE1 using 68232-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC30A antibody (68232-1-Ig, Clone: 1A11B3 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, green).

TTC30A Monoclonal antibody

TTC30A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68232-1-Ig

产品说明书

CloneNo. 1A11B3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, ELISA

别名

FLJ13946, FLJ77601, TPR repeat protein 30A, TTC30A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, rat testis tissue, HeLa cells, HEK-293 cells, mouse testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68232-1-Ig targets TTC30A in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag18361

Product name: Recombinant human TTC30A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-342 aa of BC042848

Sequence: MAGLSGAQIPDGEFTALVYRLIRDARYAEAVQLLGRELQRSPRSRAGLSLLGYCYYRLQEFALAAECYEQLGQLHPELEQYRLYQAQALYKACLYPEATRVAFLLLDNPAYHSRVLRLQAAIKYSEGDLPGSRSLVEQLLSGEGGEESGGDNETDGQVNLGCLLYKEGQYEAACSKFSATLQASGYQPDLSYNLALAYYSSRQYASALKHIAEIIERGIRQHPELGVGMTTEGFDVRSVGNTLVLHQTALVEAFNLKAAIEYQLRNYEVAQETLTDMPPRAEEELDPVTLHNQALMNMDARPTEGFEKLQFLLQQNPFPPETFGNLLLLYCKYEYFDLAADV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 30A
别名 FLJ13946, FLJ77601, TPR repeat protein 30A, TTC30A
计算分子量 665 aa, 76 kDa
观测分子量 72-76 kDa
GenBank蛋白编号BC042848
基因名称 TTC30A
Gene ID (NCBI) 92104
RRIDAB_2935319
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ86WT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tetratricopeptide repeat protein 30A (TTC30A) as a component of ciliary segmentation, may be essential for cartilage differentiation and renal tubulogenesis (PMID: 34548398). It is required for polyglutamylation of axonemal tubulin and plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.

实验方案

Product Specific Protocols
IF protocol for TTC30A antibody 68232-1-IgDownload protocol
WB protocol for TTC30A antibody 68232-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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