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验证数据展示

  • WB analysis using 28216-1-AP

    WB analysis using 28216-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28216-1-AP (TTC12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 28216-1-AP

    IF Staining of A549 using 28216-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 28216-1-AP (TTC12 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lung cancer using 28216-1-AP

    IHC staining of human lung cancer using 28216-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28216-1-AP (TTC12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TTC12 Polyclonal antibody

TTC12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28216-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

FLJ13859, FLJ20535, TPARM, TPR repeat protein 12, TTC12

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U-251 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28216-1-AP targets TTC12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag28199

Product name: Recombinant human TTC12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-257 aa of BC032355

Sequence: MDADKEKDLQKFLKNVDEISNLIQEMNSDDPVVQQKAVLETEKRLLLMEEDQEEDECRTTLNKTMISPPQTALKSAEEINSEAFLASVEKDAKERAKRRRENKVLADALKEKGNEAFAEGNYETAILRYSEGLEKLKDMKVLYTNRAQAYMKLEDYEKALVDCEWALKCDEKCTKAYFHMGKANLALKNYSVSRECYKKILEINPKLQTQVKGYLNQVDLQEKADLQEKEAHELLDSGKNTAVTTKNLLETLSKPDQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 12
别名 FLJ13859, FLJ20535, TPARM, TPR repeat protein 12, TTC12
计算分子量 79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC032355
基因名称 TTC12
Gene ID (NCBI) 54970
RRIDAB_2881092
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H892
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TTC12 antibody 28216-1-APDownload protocol
IHC protocol for TTC12 antibody 28216-1-APDownload protocol
WB protocol for TTC12 antibody 28216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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