验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human kidney tissue, liver tissue, HeLa cells, HepG2 cells, mouse kidney, rat kidney |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TST Ready-To-Use IHC Kit. TST primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
产品信息
16311-1-AP targets TST in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TST fusion protein Ag9346 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thiosulfate sulfurtransferase (rhodanese) |
| 别名 | Rhodanese, RDS, EC:2.8.1.1 |
| 计算分子量 | 297 aa, 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC010148 |
| 基因名称 | TST |
| Gene ID (NCBI) | 7263 |
| RRID | AB_2211217 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16762 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TST antibody 16311-1-AP | Download protocol |
| IHC protocol for TST antibody 16311-1-AP | Download protocol |
| IP protocol for TST antibody 16311-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Mol Med CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome. | ||
Antioxid Redox Signal The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS(-) Production and Evidence of Pancreatic and Lung Toxicity. | ||
Hum Mol Genet Coenzyme Q10 modulates sulfide metabolism and links the mitochondrial respiratory chain to pathways associated to one carbon metabolism. | ||
Biochem Pharmacol Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis. | ||
Amino Acids Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate. |
