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验证数据展示

  • IHC staining of human gliomas using 11040-1-AP

    IHC staining of human gliomas using 11040-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11040-1-AP (TSNAX antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of HeLa using 11040-1-AP

    IP experiment of HeLa using 11040-1-AP

    IP result of anti-TSNAX (IP:11040-1-AP, 4ug; Detection:11040-1-AP 1:300) with HeLa cells lysate 2400ug.

  • WB analysis using 11040-1-AP

    WB analysis using 11040-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11040-1-AP (TSNAX antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 11040-1-AP

    IF Staining of MCF-7 using 11040-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TSNAX antibody (11040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TSNAX Polyclonal antibody

TSNAX Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11040-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

translin associated factor X, Translin associated protein X, Translin-associated factor X, Translin-associated protein X, TRAX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11040-1-AP targets TSNAX in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1507

Product name: Recombinant human TSNAX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-290 aa of BC011797

Sequence: MSNKEGSGGFRKRKHDNFPHNQRREGKDVNSSSPVMLAFKSFQQELDARHDKYERLVKLSRDITVESKRTIFLLHRITSAPDMEDILTESEIKLDGVRQKIFQVAQELSGEDMHQFHRAITTGLQEYVEAVSFQHFIKTRSLISMDEINKQLIFTTEDNGKENKTPSSDAQDKQFGTWRLRVTPVDYLLGVADLTGELMRMCINSVGNGDIDTPFEVSQFLRQVYDGFSFIGNTGPYEVSKKLYTLKQSLAKVENACYALKVRGSEIPKHMLADVFSVKTEMIDQEEGIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 translin-associated factor X
别名 translin associated factor X, Translin associated protein X, Translin-associated factor X, Translin-associated protein X, TRAX
计算分子量 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC011797
基因名称 TSNAX
Gene ID (NCBI) 7257
RRIDAB_2303821
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99598
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSNAX antibody 11040-1-APDownload protocol
IHC protocol for TSNAX antibody 11040-1-APDownload protocol
IF protocol for TSNAX antibody 11040-1-APDownload protocol
IP protocol for TSNAX antibody 11040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols