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验证数据展示

  • WB analysis of Jurkat using 55426-1-AP

    WB analysis of Jurkat using 55426-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 55426-1-AP

    IP experiment of MCF-7 using 55426-1-AP

    IP result of anti-TSN (IP:55426-1-AP, 3ug; Detection:55426-1-AP 1:300) with MCF-7 cells lysate 1600ug.

  • IHC staining of mouse testis using 55426-1-AP

    IHC staining of mouse testis using 55426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSN antibody included
  • IHC staining of mouse brain using 55426-1-AP

    IHC staining of mouse brain using 55426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSN antibody included

TSN Polyclonal antibody

TSN Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 55426-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

REHF 1, translin, TRSLN, TSN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TSN Ready-To-Use IHC Kit. TSN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55426-1-AP targets TSN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 translin
别名 REHF 1, translin, TRSLN, TSN
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号NM_004622
基因名称 TSN
Gene ID (NCBI) 7247
RRIDAB_2881326
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15631
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSN, also named as Translin or Component 3 of promoter of RISC, is a 228 amino acid protein, which belongs to the translin family. TSN as a DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translations, mostly is involved in immunoglobulin T-cell receptor gene segments. TSN May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.

实验方案

Product Specific Protocols
WB protocol for TSN antibody 55426-1-APDownload protocol
IHC protocol for TSN antibody 55426-1-APDownload protocol
IP protocol for TSN antibody 55426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols