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验证数据展示

  • WB analysis of mouse liver using 12370-1-AP (same clone as 12370-1-PBS)

    WB analysis of mouse liver using 12370-1-AP (same clone as 12370-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12370-1-AP (TSKU Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12370-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 12370-1-AP (same clone as 12370-1-PBS)

    IHC staining of human colon cancer using 12370-1-AP (same clone as 12370-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12370-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 12370-1-AP (same clone as 12370-1-PBS)

    IHC staining of human colon cancer using 12370-1-AP (same clone as 12370-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12370-1-PBS in a different storage buffer formulation.

TSKU Polyclonal antibody, PBS Only

TSKU Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12370-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12370-1-PBS targets TSKU in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3054

Product name: Recombinant human TSKU protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-353 aa of BC020975

Sequence: MPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDCSGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLAVIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFSGLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPKVALHCVDTRDSAARGPTIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tsukushin
别名
计算分子量 353 aa, 38 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC020975
基因名称 TSKU
Gene ID (NCBI) 25987
RRIDAB_2877851
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WUA8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (PMID: 16884925). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted protein that contains nine LRR, which has a calculated molecular mass of 38kDa and an apparent molecular mass of 40-50kDa cause of the N-glycosylation. In chick embryos, C-TSK is a unique BMP inhibitor that forms a ternary complex with BMP and chordin and is involved in the regulation of primitive streak and node formation. C-TSK migrates as a band of 47 kDa in SDS-PAGE and N-glycosidase F treatment reduces the size to 41 kDa (PMID: 15363410).

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