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验证数据展示

  • WB analysis of mouse liver using 12370-1-AP

    WB analysis of mouse liver using 12370-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12370-1-AP (TSKU Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12370-1-AP

    IHC staining of human colon cancer using 12370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12370-1-AP

    IHC staining of human colon cancer using 12370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TSKU Polyclonal antibody

TSKU Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12370-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

E2 induced gene 4 protein, E2IG4, LRRC54, TSK, TSKU, Tsukushi, tsukushin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, mouse colon tissue, mouse pancreas tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12370-1-AP targets TSKU in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TSKU fusion protein Ag3054 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tsukushin
别名 E2 induced gene 4 protein, E2IG4, LRRC54, TSK, TSKU, Tsukushi, tsukushin
计算分子量 353 aa, 38 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC020975
基因名称 TSKU
Gene ID (NCBI) 25987
RRIDAB_2877851
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WUA8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (PMID: 16884925). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted protein that contains nine LRR, which has a calculated molecular mass of 38kDa and an apparent molecular mass of 40-50kDa cause of the N-glycosylation. In chick embryos, C-TSK is a unique BMP inhibitor that forms a ternary complex with BMP and chordin and is involved in the regulation of primitive streak and node formation. C-TSK migrates as a band of 47 kDa in SDS-PAGE and N-glycosidase F treatment reduces the size to 41 kDa (PMID: 15363410).

实验方案

Product Specific Protocols
WB protocol for TSKU antibody 12370-1-APDownload protocol
IHC protocol for TSKU antibody 12370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols