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TSEPA Polyclonal antibody

TSEPA Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 27415-1-AP

Print datasheet

Synonyms

C6orf190, C6orf207, Protein THEMIS, THEMIS, TSEPA

验证数据展示

  • WB analysis of Jurkat using 27415-1-AP

    WB analysis of Jurkat using 27415-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27415-1-AP (TSEPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27415-1-AP

    IHC staining of human tonsillitis using 27415-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27415-1-AP

    IHC staining of human tonsillitis using 27415-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27415-1-AP targets TSEPA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen TSEPA fusion protein Ag26387 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thymocyte selection pathway associated
Synonyms C6orf190, C6orf207, Protein THEMIS, THEMIS, TSEPA
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC130516
Gene Symbol TSEPA
Gene ID (NCBI) 387357
RRIDAB_2880866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1K5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSEPA antibody 27415-1-APDownload protocol
IHC protocol for TSEPA antibody 27415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols