验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
27415-1-AP targets THEMIS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | THEMIS fusion protein Ag26387 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thymocyte selection pathway associated |
| 别名 | TSEPA, Thymocyte-expressed molecule involved in selection, Protein THEMIS, C6orf207, C6orf190 |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC130516 |
| 基因名称 | TSEPA |
| Gene ID (NCBI) | 387357 |
| RRID | AB_2880866 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N1K5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The T cell lineage-restricted protein THEMIS, also called C6orf190 and C6orf207, plays a critical role in T cell development at the positive selection stage (PMID: 37159521). THEMIS plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. It is required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells and regulates T-cell development through T-cell antigen receptor (TCR) signaling (PMID: 28697966, 28250424). It can also promote the proliferative response of CD8+ T cells to low-affinity ligands (PMID: 31932808).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for THEMIS antibody 27415-1-AP | Download protocol |
| IHC protocol for THEMIS antibody 27415-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
