验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, human brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
13103-2-AP targets TSEN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | TSEN2 fusion protein Ag3742 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tRNA splicing endonuclease 2 homolog (S. cerevisiae) |
| 别名 | tRNA-intron endonuclease Sen2, SEN2L, SEN2, PCH2B, HsSen2 |
| 计算分子量 | 465 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC019582 |
| 基因名称 | TSEN2 |
| Gene ID (NCBI) | 80746 |
| RRID | AB_2272213 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NCE0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSEN2, also named as SEN2, belongs to the tRNA-intron endonuclease family. TSEN2 is a metal ion independent nuclease that cleave the 5' splice site (PMID: 32476018). TSEN2 has 5 isoforms with the molecular mass of 45-53 kDa. TSEN2 is involved in the formation of a human endonuclease complex whose function is the removal of introns from pre-tRNA (PMID: 16595010).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSEN2 antibody 13103-2-AP | Download protocol |
| IF protocol for TSEN2 antibody 13103-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration. | ||
Am J Hum Genet Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly. | ||
Genetics An unknown essential function of tRNA splicing endonuclease is linked to the integrated stress response and intron debranching
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