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验证数据展示

  • FC experiment of THP-1 using 98690-1-RR

    FC experiment of THP-1 using 98690-1-RR

    1x10^6 THP-1 cells were surface stained with 0.25 ug Anti-Human TRPV4 Rabbit RecAb (98690-1-RR, Clone:251361G8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • IHC staining of human colon using 98690-1-RR

    IHC staining of human colon using 98690-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98690-1-RR (TRPV4 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 98690-1-RR

    IHC staining of human colon using 98690-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98690-1-RR (TRPV4 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • SEC-HPLC of 98690-1-RR

    SEC-HPLC of 98690-1-RR

    The purity of Human TRPV4 Recombinant monoclonal antibody (Cat. No. 98688-2-RR) was greater than 95% as determined by SEC-HPLC.

Anti-Human TRPV4 Rabbit Recombinant Antibody

TRPV4 Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98690-1-RR

产品说明书

CloneNo. 251361G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC

别名

Osm 9 like TRP channel 4, Osm-9-like TRP channel 4, OTRPC4, TRP12, Vanilloid receptor-like channel 2

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98690-1-RR targets TRPV4 in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily V, member 4
别名 Osm 9 like TRP channel 4, Osm-9-like TRP channel 4, OTRPC4, TRP12, Vanilloid receptor-like channel 2
计算分子量 98 kDa
GenBank蛋白编号BC117426
基因名称 TRPV4
Gene ID (NCBI) 59341
RRIDAB_3746278
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9HBA0
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TRPV4, also called VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.

实验方案

Product Specific Protocols
FC protocol for TRPV4 antibody 98690-1-RRDownload protocol
IHC protocol for TRPV4 antibody 98690-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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