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验证数据展示

  • WB analysis using 83062-4-RR

    WB analysis using 83062-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83062-4-RR (TRPS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 83062-4-RR

    FC experiment of MCF-7 using 83062-4-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TRPS1 Recombinant antibody (83062-4-RR, Clone:230051F10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of human breast cancer using 83062-4-RR

    IHC staining of human breast cancer using 83062-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 83062-4-RR (TRPS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83062-4-RR

    Affinity and Kinetic Characterization of 83062-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83062-4-RR against Human TRPS1 were performed. The affinity constant is 88.0 pM.

TRPS1 Recombinant monoclonal antibody

TRPS1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83062-4-RR

产品说明书

CloneNo. 230051F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79, 230051F10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83062-4-RR targets TRPS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33959

Product name: Recombinant human TRPS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 9-358 aa of BC125020

Sequence: LRNVASEGEGQILEPIGTESKVSGKNKEFSADQMSENTDQSDAAELNHKEEHSLHVQDPSSSSKKDLKSAVLSEKAGFNYESPSKGGNFPSFPHDEVTDRNMLAFSSPAAGGVCEPLKSPQRAEADDPQDMACTPSGDSLETKEDQKMSPKATEETGQAQSGQANCQGLSPVSVASKNPQVPSDGGVRLNKSKTDLLVNDNPDPAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFSHSKDFQKVNRSVFSGVLQDINSSRPVLLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMGNSSTELEQHFLQTH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trichorhinophalangeal syndrome I
别名 Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79, 230051F10
计算分子量 1294 aa, 143 kDa
观测分子量150 kDa
GenBank蛋白编号BC125020
基因名称 TRPS1
Gene ID (NCBI) 7227
RRIDAB_3670788
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHF7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains.

实验方案

Product Specific Protocols
IHC protocol for TRPS1 antibody 83062-4-RRDownload protocol
WB protocol for TRPS1 antibody 83062-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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