验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HeLa cells, PANC-1 cells, SK-BR-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86735-2-RR targets TROAP in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag4562 Product name: Recombinant human TROAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 428-778 aa of BC032583 Sequence: QEVKIQRIGILQQLLRQEVEGLVGGQCVPLNGGSSLDMVELQPLLTEISRTLNATEHNSGTSHLPGLLKHSGLPKPCLPEECGEPQPCPPAEPGPPEAFCRSEPEIPEPSLQEQLEVPEPYPPAEPRPLESCCRSEPEIPESSRQEQLEVPEPCPPAEPRPLESYCRIEPEIPESSRQEQLEVPEPCPPAEPGPLQPSTQGQSGPPGPCPRVELGASEPCTLEHRSLESSLPPCCSQWAPATTSLIFSSQHPLCASPPICSLQSLRPPAGQAGLSNLAPRTLALRERLKSCLTAIHCFHEARLDDECAFYTSRAPPSGPTRVCTNPVATLLEWQDALCFIPVGSAAPQGSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | trophinin associated protein (tastin) |
| 别名 | Tastin, Trophinin assisting protein, Trophinin associated protein, Trophinin-assisting protein, Trophinin-associated protein |
| 计算分子量 | 778 aa, 84 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC032583 |
| 基因名称 | TROAP |
| Gene ID (NCBI) | 10024 |
| RRID | AB_3745091 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q12815 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TROAP antibody 86735-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
