×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86735-2-RR (same clone as 86735-2-PBS)

    WB analysis using 86735-2-RR (same clone as 86735-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86735-2-RR (TROAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86735-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02627-1

    Cytometric bead array sample test of MP02627-1

    The mean TROAP concentration was determined to be 0.7 ng/mL in HCT 116 cell extract based on a 1.3 mg/mL extract load, 1.1 ng/mL in PC-3 cell extract based on a 1.3 mg/mL extract load.

  • Cytometric bead array standard curve of MP02627-1

    Cytometric bead array standard curve of MP02627-1

    Cytometric bead array standard curve of MP02627-1, TROAP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86735-2-PBS. Detection antibody: 86735-1-PBS. Standard: Ag4562. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 86735-2-RR

    Affinity and Kinetic Characterization of 86735-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86735-2-RR against Human TROAP were performed. The affinity constant is 89.2 pM.

TROAP Recombinant monoclonal antibody, PBS Only (Capture)

TROAP Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 86735-2-PBS

产品说明书

CloneNo. 251538B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

Tastin, Trophinin assisting protein, Trophinin associated protein, Trophinin-assisting protein, Trophinin-associated protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TROAP Ready-To-Use IHC Kit. TROAP primary antibody included.

产品信息

86735-2-PBS targets TROAP as part of a matched antibody pair:

MP02627-1: 86735-2-PBS capture and 86735-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4562

Product name: Recombinant human TROAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 428-778 aa of BC032583

Sequence: QEVKIQRIGILQQLLRQEVEGLVGGQCVPLNGGSSLDMVELQPLLTEISRTLNATEHNSGTSHLPGLLKHSGLPKPCLPEECGEPQPCPPAEPGPPEAFCRSEPEIPEPSLQEQLEVPEPYPPAEPRPLESCCRSEPEIPESSRQEQLEVPEPCPPAEPRPLESYCRIEPEIPESSRQEQLEVPEPCPPAEPGPLQPSTQGQSGPPGPCPRVELGASEPCTLEHRSLESSLPPCCSQWAPATTSLIFSSQHPLCASPPICSLQSLRPPAGQAGLSNLAPRTLALRERLKSCLTAIHCFHEARLDDECAFYTSRAPPSGPTRVCTNPVATLLEWQDALCFIPVGSAAPQGSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trophinin associated protein (tastin)
别名 Tastin, Trophinin assisting protein, Trophinin associated protein, Trophinin-assisting protein, Trophinin-associated protein
计算分子量 778 aa, 84 kDa
观测分子量120 kDa
GenBank蛋白编号BC032583
基因名称 TROAP
Gene ID (NCBI) 10024
RRIDAB_3745091
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12815
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939).

||
New chat

Able™

正在加载，请稍候...