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验证数据展示

  • IHC staining of mouse brain using 83410-4-RR

    IHC staining of mouse brain using 83410-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83410-4-RR (TRNP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83410-4-RR

    IHC staining of mouse brain using 83410-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83410-4-RR (TRNP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 83410-4-RR

    IF Staining of mouse brain using 83410-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRNP1 antibody (83410-4-RR, Clone: 240331B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83410-4-RR

    IF Staining of HeLa using 83410-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRNP1 antibody (83410-4-RR, Clone: 240331B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83410-4-RR

    FC experiment of HeLa using 83410-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRNP1 Recombinant antibody (83410-4-RR, Clone:240331B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83410-4-RR

    Affinity and Kinetic Characterization of 83410-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83410-4-RR against Human TRNP1 were performed. The affinity constant is 2.5 nM.

TRNP1 Recombinant antibody

TRNP1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 83410-4-RR

产品说明书

CloneNo. 240331B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, IF-P, ELISA

别名

TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331B6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83410-4-RR targets TRNP1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 TRNP1 fusion protein Ag22504 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TMF1-regulated nuclear protein 1
别名 TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331B6
计算分子量 227 aa, 23 kDa
GenBank蛋白编号BC069216
基因名称 TRNP1
Gene ID (NCBI) 388610
RRIDAB_3671058
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6NT89
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.

实验方案

Product Specific Protocols
IHC protocol for TRNP1 antibody 83410-4-RRDownload protocol
IF protocol for TRNP1 antibody 83410-4-RRDownload protocol
FC protocol for TRNP1 antibody 83410-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols