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验证数据展示

  • IF Staining of HeLa using CL488-83843-5

    IF Staining of HeLa using CL488-83843-5

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TRMT6 antibody (CL488-83843-5, Clone: 240911C5 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated TRMT6 Recombinant monoclonal antibody

TRMT6 Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-83843-5

产品说明书

CloneNo. 240911C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

240911C5, CGI 09, CGI-09, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, TRM6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRMT6 Ready-To-Use IHC Kit. TRMT6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83843-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83843-5 targets TRMT6 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag10176

Product name: Recombinant human TRMT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-497 aa of BC001262

Sequence: MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tRNA methyltransferase 6 homolog (S. cerevisiae)
别名 240911C5, CGI 09, CGI-09, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, TRM6
计算分子量 56 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC001262
基因名称 TRMT6
Gene ID (NCBI) 51605
RRIDAB_3673313
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UJA5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRMT6 antibody CL488-83843-5Download protocol
Standard Protocols
Click here to view our Standard Protocols